NAD+: The Cellular Power Plant Guide

What is NAD+?

NAD+ (Nicotinamide Adenine Dinucleotide) is a vital coenzyme found in every cell of your body. It's often called the "cellular powerhouse" because it plays a critical role in:

• Energy production - Converting nutrients into ATP (cellular fuel)
• DNA repair - Fixing damaged genetic material
• Cellular regeneration - Activating sirtuins (longevity enzymes)
• Mitochondrial function - Powering your cells' energy factories

Why NAD+ Matters

NAD+ levels naturally decline with age. By age 50, most people have about half the NAD+ they had in their youth. This decline is linked to:

• Decreased energy and mental clarity
• Slower recovery from exercise
• Accelerated aging
• Reduced metabolic function
• Cognitive decline

Why Inject NAD+?

Unlike oral supplements (NMN, NR), NAD+ injections:

• Bypass the digestive system for direct absorption
• Higher bioavailability - more reaches your cells
• Faster results - effects felt within days
• More efficient - requires lower total doses

Benefits of NAD+ Therapy

Energy & Performance

• Enhanced mental clarity and focus
• Improved physical stamina
• Better exercise recovery
• Reduced brain fog

Anti-Aging

• DNA repair and cellular regeneration
• Improved skin health and appearance
• Activation of longevity pathways (sirtuins)
• Protection against oxidative stress

Metabolic Health

• Enhanced insulin sensitivity
• Improved lipid metabolism
• Better nutrient utilization
• Weight management support

Cognitive Function

• Improved memory and concentration
• Reduced age-related cognitive decline
• Enhanced neuroplasticity
• Better mood regulation

Reconstitution Guide

For a 500mg Vial

Option 1 (Standard): Add 5mL bacteriostatic water = 100mg/mL

Option 2 (Concentrated): Add 3mL bacteriostatic water = 166.7mg/mL

Step-by-Step Mixing

1. Allow vial to reach room temperature (do not heat)
2. Clean vial top with alcohol swab
3. Draw bacteriostatic water into a sterile syringe
4. Inject slowly down the vial wall - avoid direct stream onto powder
5. Swirl gently until completely dissolved - DO NOT SHAKE
6. Label with date and concentration
7. Refrigerate immediately

Storage After Reconstitution

• Refrigerate at 36-46°F (2-8°C)
• Use within 30 days of reconstitution
• Protect from light
• Never freeze reconstituted solution
• Discard if solution becomes cloudy or discolored

Dosing Protocol

Starting Protocol (First 4 Weeks)

| Week | Dose | Frequency |

|------|------|-----------|

| 1 | 50mg | Once weekly |

| 2 | 50mg | Once weekly |

| 3 | 75mg | Once weekly |

| 4 | 100mg | Once weekly |

Loading Phase (Optional - for faster results)

For those wanting to rapidly restore depleted NAD+ levels:

• 100-200mg daily for 7-10 consecutive days
• Then transition to maintenance dosing

Maintenance Dosing by Goal

| Goal | Dose | Frequency |

|------|------|-----------|

| General Wellness | 50-100mg | Weekly or bi-weekly |

| Anti-Aging | 100mg | 1-2x weekly |

| Athletic Recovery | 100-150mg | 2-3x weekly (during training) |

| Cognitive Enhancement | 100mg | 2x weekly |

Maximum Dosing

• Do not exceed 300mg per week for home use without medical supervision
• Single doses above 150mg may be split into two injection sites

Injection Guide

Best Time to Inject: Morning

NAD+ boosts cellular energy production, so morning injections are ideal:

• Provides energy throughout the day
• Won't interfere with sleep
• Aligns with natural circadian rhythms

Injection Sites (Rotate Weekly)

• Abdomen - 2 inches from navel (most common)
• Thigh - Front or outer
• Upper arm - Back of arm

Injection Technique

1. Clean injection site with alcohol swab
2. Pinch a fold of skin
3. Insert needle at 45-90 degree angle
4. Inject slowly - NAD+ can cause a brief stinging sensation
5. Hold for 5-10 seconds before withdrawing
6. Apply gentle pressure (don't rub)

Tips for Comfortable Injections

• Allow refrigerated solution to warm slightly before injecting
• Inject slowly to minimize discomfort
• Rotate sites to prevent tissue irritation
• If burning occurs, it's normal and temporary (1-2 minutes)

What to Expect

First Few Days

• Possible mild nausea or flushing (temporary)
• Some feel an energy boost immediately
• Others notice improved sleep quality

Week 1-2

• Increased mental clarity
• Better focus and concentration
• Improved energy levels
• Enhanced mood

Week 4-8

• Noticeable improvements in stamina
• Better exercise recovery
• Skin quality improvements
• More consistent energy throughout the day

Long-Term (3-6 months)

• Significant anti-aging benefits
• Sustained cognitive improvements
• Better overall metabolic health
• Enhanced longevity markers

Managing Side Effects

Common Side Effects (Usually Mild)

• Brief stinging at injection site
• Temporary flushing or warmth
• Mild nausea (especially at higher doses)
• Headache (usually from dehydration)

How to Minimize Side Effects

• Start with lower doses and increase gradually
• Stay well hydrated - drink plenty of water
• Inject slowly to reduce stinging
• Take with food if nausea occurs
• Avoid evening dosing to prevent sleep disruption

When to Reduce Dose

If you experience persistent:

• Insomnia or sleep disturbances
• Anxiety or restlessness
• Fatigue (paradoxically, too much can cause this)
• Digestive upset

Stacking NAD+ with Other Peptides

NAD+ works synergistically with other compounds:

Recommended Stacks

For Anti-Aging:

• NAD+ + GHK-Cu (skin and cellular health)
• NAD+ + Epitalon (telomere support)

For Performance:

• NAD+ + CJC-1295/Ipamorelin (GH optimization)
• NAD+ + BPC-157 (recovery)

For Weight Loss:

• NAD+ + Retatrutide (metabolic enhancement)
• NAD+ + AOD-9604 (fat metabolism)

Why NAD+ Enhances Other Peptides

As mentioned in the research: "Without enough NAD+, your body can't efficiently use the signals sent by peptides like CJC-1295 or BPC-157." Think of NAD+ as the battery that powers all other cellular processes.

Important Considerations

Contraindications

NAD+ may not be suitable for those with:

• Known allergy to NAD+ or its components
• Severe liver or kidney impairment
• History of certain cancers
• Cardiovascular disease or bleeding disorders

Consult Your Healthcare Provider If:

• You're taking medications for diabetes
• You have a history of mental health conditions
• You're pregnant or breastfeeding
• You have any chronic medical conditions

Cycling Recommendations

Suggested Protocol

• 8-12 weeks on at maintenance dose
• 2-4 weeks off or reduced frequency
• Resume as needed

Why Cycle?

While NAD+ is naturally occurring, cycling helps:

• Prevent tolerance
• Allow natural production to normalize
• Assess your baseline needs
• Optimize long-term results

Research Disclaimer

NAD+ injections are prepared by compounding pharmacies and are not FDA-approved medications. The information provided is for educational purposes only. Always consult with a qualified healthcare provider before starting NAD+ therapy. Individual results may vary based on age, health status, and other factors.